Budget Hotel

The Advantages and Disadvantages of 7 Days Inn As a typical budget hotel, Seven Days Group Holdings Limited established in 2005 and now, more than 1000 Seven Days Inn have scattered all around the China (Zhang, 2012). I registered to be a member of 7 Days Inn two years age, and have already lodged in three branch stores in different cities during my travels. Budget hotels are considered as 1 star hotel because they provide lodging at cheaper price varies from 30$-40$ per room per night.The main service is providing guest rooms with limited amenities for general tourists and business travelers(Song, 2009). In the last few years, budget hotel has demonstrated its huge market potential, especially within the key consumer groups, like backpackers and university students. Being one of the top ten budget hotels in China, 7 Days Inn has its obvious advantages, such as various reservation systems, convenient hotel locations and enthusiastic services. The primary reason for customers choosing Seven Days Inn is that it provides a complete hotel reservations system.Hotel reservation system, commonly known as a central reservation system (CRS) is a computerized system that stores and distributes information of a hotel, resort, or other lodging facilities (Zhang, 2012). A complete reservation system is important because it is assistance for hotel staffs to manage all of their online marketing and sales, where they can upload their rates and availabilities to be seen by all customers. And for the customers, they can finish their reservations through multiple channels, like internet, telephone and e-mail.Thanks to the 7 Days Inn’s complete reservation system, I could still book a room in time with my mobile phone after I suddenly changed my travel plan in Zhuhai. The second advantage of 7 Days Inn is the convenient location. During my traveling, I found it easy to get to the locations of the branch stores I had booked, because they were either near bus stations or the landmarks of the districts. Different kinds of customers choose 7 Days Inn for different purposes, some for vacations, while others for business. No matter for what purposes, locations of the hotels will surely play a big role in the overall enjoyment of the trip.That's because a well-located hotel will significantly cut down the amount of time you waste in traffic or trying to navigate an unfamiliar place. As a customer-friendly budget hotel, nearly 35% of the 7 Days Inn runs shuttles to and from popular sightseeing destinations, and the rest of its branch stores mainly locate near the metro stations or bus stations (Liu, 2008). At that point, the customers will save money as well as time on cab fare or public transportation. All the staffs trying their best to provide enthusiastic services are the most prominent advantage of the 7 Days Inn.Service quality has been reported as having apparent relationship to costs, profitability, customer satisfaction, customer retention, behaviora l intention, and positive word-of-mouth (Boltan, 1991). To provide high quality service, all staffs in 7 Days Inn are required to wear clean uniform and satisfy customers’ requirements with smiling faces to the greatest extent. Besides, high service quality also has strategic benefits of contributing to market-share and return on investment, as well as in lowering manufacturing costs and improving productivity.Accordingly, in 2011, 7 Days Inn’s annual revenue growth rate increased 28%, which illustrates the importance of high service quality (Li, 2012). Although Seven Days Inn has three aforementioned advantages, at the same time, its reputation is also devalued at a certain extent by its disadvantages, such as the deficient company website with limited information and obsolete equipments in some guest rooms. Firstly, 7 Days Inn’s company website is underdeveloped, which just with some limited or outdated information.A company website is a fairly inexpensive bus iness tool that can serve a purpose for both the company and its clients. With a thorough company website, 7 Days Inn can feature a complete electronic version of its catalog, in which describe all its products and services in greater details. For its clients, they will have the convenience of reading about the service and updated information on their own time. 7 Days Inn already had a website, but as a permanent member, I only use it for my reservation. Most of the customers can not find some more interesting and eye-catching information.Besides presenting its hotel rate and some basic hotel introductions, to well develop its company website, the 7 Days Inn’s webmasters should take measures to perfect its company website, like encouraging all the staffs and customers to publish their ideas and experiences of lodging in 7 Days Inn. On the other hand, obsolete and deficient equipments in some guest rooms also make the customers unsatisfied with 7 Days Inn. I once complained th e small and obsolete TV set in my guest room, because I even could not find CCTV-NEWS channel.Among all the problems about deficient and obsolete equipments, the dullish and yellow lighting in some branch stores is most annoying. Lighting, whose aim is to temporarily receive guests and revivify the staffs, has to be designed for different purposes in different areas. However, in two branch stores that I lodged in, the lightings in corridors were so dim during days and nights, while the lighting color and intensity in the guest rooms was unchangeable. In a hotel, during the day, people are attracted by the interior design, by furnishings, shapes and colors.But at night the hotel has a second dimension, since the guest needs to stay in a safe, comfortable and protected atmosphere, but even relaxing, clean and efficient (Cui, 2007). So the lighting system in 7 Days Inn can be perfected by installing new type of lighting which can change its color and intensity, especially in the recept ions and guest rooms. The disadvantages I mentioned above have been complained by some other customers according to the evaluation records in its website. So, it is high time for the managements of 7 Days Inn to take actions. In 2011, 7 Days Inn’s market share was 12. 3%, the second among all the budget hotels in China. During the previous three quarters, it opened 838 branch stores, 95 more when compared with the year earlier (Li, 2012). With its rapid development, 7 Days Inn will surely occupy larger market shares. But, it has to insure the environment and services quality in every branch store. Nevertheless, improving its disadvantages is the main task for managements. References Boltan, R. N. (1991). A Longitudinal Analysis of the Impact of Service Changes on Customer Attitude. Journal of Marketing, 20(1), 1-25. Retrieved June 16, 2012, from http://www. jstor. rg/stable/25061184? . Cui, D. D. (2007). An Analysis of the Developing Prospects and Strategies of Chinese Econom y Hotel from 2000 to 2007. Journal of the Postgraduate of Zhongnan University of Economics and Laws, 5(3), 5-11. Retrieved June 16, 2012, from http;//dlib4. ehttp://dlib4. edu. cnki. net/kns50/detail. aspx? dbname=CJFDTEMP=ZNCZ200706018. Li, D. (2012). Analysis on Financing Development of Economic Hotel in Capital Market. Journal of Tourism College of Zhejiang, 9(3), 13-20. Retrieved June 16, 2012, from http://dlib4. edu. cnki. net/kns50/detail/=CJFDTEMP=LYXY20080212. Liu, M. Q.

International Trade & Finance Speech Essay

International trade is essentially when two or more countries exchange goods and services. Many countries export their goods and services to other countries and in turn, they can also import goods and services from other countries to into their own. Advancements with technology have made it a lot easier for international trade to take place. Communication between countries is a good example. Communicating has vastly improved and helped to simplify the trading process. Some technologically advanced countries, like Japan and China have bountiful natural resources and that has a heavy impact on us. The United States is one of the largest contributors to international trade. Our GDP (Gross Domestic Product), is greatly impacted due to being huge import consumers. The United States relies heavily on products from other countries and we import much more than we export. Not only does this impact our GDP by lowering it as we import more than export, is also has an impact on our domestic markets because we are buying more from other countries. In regard to Foreign Exchange Rates, it is very important to know how they are determined. Considering economic growth within a country is important, governments can make certain that fiscal and monetary policies are in place to ensure that growth continues. Due to the goods and services that are traded between different countries around the world, there are foreign exchange rate payments that are required to be paid. You will see the foreign exchange rate differ from country to country. What happens when there is a surplus of imports brought into the U.S.? Cite a specific example of a product with an import surplus and the impact that it has on the U.S. Businesses and Consumers involved. First, it is important to try and keep imports and exports balanced. However, when one exceeds the other, it is called a surplus. Having a surplus of imports can create a lower price for the consumer, and have a positive effect on the employment rate of the country where the product was obtained. Seafood is a good example of a product with surplus. There are several states, mainly coastal states that have fishing boats out at sea for many months catching lobster, fish, crabs, etc. The seafood that is collected is used for both domestic sales and international export. This allows for several options when selecting fish, etc at the grocery store. It also increases your selection when out dining at a restaurant. A downfall could be that a surplus of seafood, fish, etc could result in a declining need for domestic fisherman to work and this could cause layoffs and cutbacks. What are the effects of International Trade to GDP, Domestic markets and University Students? GDP stands for Gross Domestic Product and international trade. GDP is the market value of all final goods and services produced in an economy in a one year period. (Colander, 2010) International trade is the exchange of goods and services between two or more countries. International trade greatly affects the GDP due to the fact that is we are able to have goods produced outside of the United States and the imported for sale at a cheaper cost than if we were to produce here, the consumer demands will increase and therefore help the GDP. Domestic markets are also affected by international trade as they have the ability to have products manufactured outside the United States at cheaper rates. This takes away from domestic manufactured product sales and could increase unemployment. On the reverse, it could affect domestic markets positively, as domestic retailers could mark up the products to consumers and keep the additional profit. International trade affects University students in a much different way. Suppose there was an increased demand for innovative ideas and qualified individuals to help keep our domestic markets alive? A University student could be a huge asset there. Also, the higher the GDP, more jobs will be available. If the GDP crashes, graduates should probably look for a job in another country. How do Government choices in regards to tariffs and quotas affect international relations and trade? Both International relations and trade are greatly affected by government decisions that are made about tariffs and quotas. Tariffs are the most familiar and most commonly used type of trade restriction. Tariffs are most often recognized as taxes here in the United States. Quotas are quantity limits that are placed on imports. These limits are decided by the government. The choices made by the government as it relates to additional fees for imports and limitations placed on the amount of imports directly affects international relations and trade. The positive side to this is that the government has the ability to control trade between themselves and other countries. This allows the United States to be picky. The ability to raise or lower tariff amounts gives us the power to possibly do business with underprivileged countries simply by lowering the tariffs as an incentive. They can also raise the tariff as a means to end relations with a country. What are foreign exchange rates? How are they determined? In regard to Foreign Exchange Rates, it is very important to know how they are determined. Considering economic growth within a country is important, governments can make certain that fiscal and monetary policies are in place to ensure that growth continues. Due to the goods and services that are traded between different countries around the world, there are foreign exchange rate payments that are required to be paid. You will see the foreign exchange rate differ from country to country. Why doesn’t the U.S. simply restrict all goods coming in from China? Why can’t the U.S. just minimize the amounts of imports coming in from all other countries? To put it simply, it is not possible for the U.S. to restrict all goods from China. This is mainly because there is an enormous trade deficit between us and China. China currently holds a very large portion of our nation’s debt and it would be a bad business decision to try and end relations when we still have a large debt owed to them. Not only do we have a large debt owed to China, the U.S. consumer has a very large demand for products that are made in China. China is one of the largest manufacturers of electronics and other luxury items, like the Iphone, Ipad, Ipod that we have come to rely upon. If we were to restrict all goods, we would not only destroy our economy, but also China’s economy. References: Colander, D.C. (2010). Macroeconomics. (8th ed). Boston, MA: Mcgraw-Hill/Irwin Trading Economics. (2012) United States Consumer Confidence. Retrieved from http://www.tradingeconomics.com/united-states/consumer-confidence.

Can negotiations occur without trust

Can negotiations occur without trust BY Richer The nature of negotiation and its process constitutes a number of various factors that leads to an effective or ineffective process and outcome. One of the primary purposes of a negotiation is to come an agreement with another party by exchanging offers and to find solutions to a common issue, â€Å"whenever we cannot achieve our objectives single-heartedly' (Thompson, 2009).Some believe that in order to conduct a negotiation, trust is an utmost important factor as negotiators depend on the information provided by the other party, on the outcomes to which the parties omit throughout the negotiation process, and on the other party to deliver the commitments (Licked, Poplin, 2013). This essay aims to discuss whether trust is a precondition for negotiation. As such, it will attempt to reach to a deeper understanding of trust in a negotiation, before understanding how it may influence the outcome, and whether a negotiation may take place be tween us and the people whom we do not trust.Examples of trust and distrust within negotiations are given, before the essay will conclude with reasons why it is impossible to successfully negotiate with people whom we do not trust. Trust is commonly defined as a confident and positive expectation of the actions of another party (Lessons, Smith, 2012). Trusting gives us the assumption that the other party would meet the expectation by considering our welfare and honoring their commitment towards the agreement.Trust in negotiations, like in any other independent relationships, is characterized by dependency and vulnerability to the other party. Trust in this case, according to Lessons, implies to the positive expectation to sought common solutions to create value, integrate interests, and find mutually beneficial solutions (Lessons, Smith, 2012). This brings us to the characteristic of an integrative negotiation, which seeks to create value and achieving joint gains. In such cases, ne gotiators are in dependence to each or one another for the sharing or exchange of information.According to Licked and Poplin, even though a negotiator may have a certain level of doubt in the other party, he or she still has to have at least a bit of trust, otherwise, it may be impossible to accept anything the other party says at face value or reach a viable agreement (Licked, Poplin, 2013). As a result, being constantly afraid and doubtful with what is being shared would not low us to take a step further. This is further aggravated by the fact that negotiators may not be able to verify the received information.Here, trust is more about acceptance rather than further scrutiny and serves as stepping-stones to have an exchange of information during the negotiation process. Without trust, we are not able to move on further to create solutions and agreements based on the information received. As such, the negotiation is impossible to proceed further due to an absence of trust. In a neg otiation, many parties tend to focus on positions, not interests. The relevance f how interests could halt a negotiation seems to interlink with the problem of having trust.If we do not trust the other party, we lack an openness to share our objectives and understand their interests in the negotiation. In an example of the story shared by (Fisher, Our, 2006), two men were in conflict in a library as one wanted the window open and the other wanted it closed. Both could not come to an agreement on how much to leave the window open. When a third party, the librarian asked why both mean wanted it closed or open, one shared that he wanted to get fresh air, but the other wanted to avoid the draft (Fisher, Our, 2006).Here, we can see how both men focused on the position, the â€Å"what†, and not each other's interests, the â€Å"why'. As such, we can see that without understanding each other's interest, both men came into conflict and could not negotiate on a solution. As a result, it then becomes virtually impossible to really identify or appreciate the problem that actually needs to be addressed (Fisher, Our, 2006). To build on this, trust is extremely important as it serves as a foundation for understanding each other's interest before working on the solutions or agreements.Without trust, both parties will not seek to understand each other, and end up being fixated on their positions due to their own interests. This prevents both parties to successfully negotiate on the issue. In order to negotiate, trust is important in order to avoid retaliation due to a conflict of views and ideas. Without trust, every solution and idea will be met with suspicion and skepticism. This lack of trust not only inhibits cooperation and successful negotiation, it may also result in retaliation that causes the escalation of conflict (Coleman, et al. 2000). In addition, according to (Kramer, 1994), this may even result n paranoid cognitions in which one may feel that he or she is subject to the other party malevolence. These perceptions may then drive him or her to the point of hyper vigilance and rumination, resulting in a faulty diagnosis of the result of working with the other party (Licked, 2006). As such, conflicts would then arise, as the negotiator would increasingly focus on his or her position, and end up reluctant to come into agreement.The negotiation will then come to an impasse as both sides end up using their negative perceptions to retaliate each other, causing the conflict to escalate beyond control. As explained above, trust seems to be indispensable in order to negotiate. However, according to a writer Bonnie Change, claiming trust is necessary for all negotiation seem to be an overstatement as the significance of trust is culture and context specific (Change, 2009). In her example of how two people may conduct a negotiation without trust, she gave a scenario of negotiation with hostage- takers.A negotiator and a hostage-taker do not kno w each other and thus, will not have any trust between them. However, both of them may still engage in a negotiation to lay down the benefits of letting the hostage go. The negotiator's main objective is to talk the hostage-taker into revealing information about him or herself, and find out what kind of concessions to make for the release of the hostage. The hostage-taker's main objective is to use the hostage to obtain what he or she needs, including the escape from the crime.The succession of the negotiation in such cases are a result of the weight of the gains and losses, depending on which side has more power to influence the outcome. For example, if the hostage were to be a high profile person, the hostage-taker would have more power to negotiate through to his or her wants. Therefore, through this example, negotiations may occur even though both parties do not trust each other. According to (Choc, 2013), should there be no trust between two parties, relying on formal legal mec hanisms such as preparing contracts are constructive ways to proceed with a negotiation.These formalized documents remind people of what they had agreed upon and serves as documentation, minimizing miscommunication that may occur if a deal is made based on fertilization. Creation of such agreements usually requires elaborate consequences and penalties for violating the terms of agreement (Licked, Poplin, 2013). These consequences of violation commonly includes penalties such as monetary compensations and mechanisms such as law and the police force will be brought in to become part of the system of enforcement, should any agreements be violated.With a contract to force people to keep to a certain agreement, trust is not required in order for a negotiation to occur. We meet new people everyday, and sometimes, negotiations may occur whether or not we know the person. Of course, trust is not immediately established. Rather, like impressions, our Judgments of trustworthiness can occur ra pidly at the start of a stagnation. This trustworthiness may result from what the negotiator has learned about the other party's reputation. For example, if I wanted to buy something for a shop owner for the first time, I will naturally exhibit skepticism in the shop owner and the quality of the goods.However, if I see a crowd at the shop with people snapping up the items, naturally, I would have the trust in the shop owner that the goods sold would be good. Here, we can see how the reputation of a person may easily create trust in us, towards the other party, even if we were to be meeting for the first time. In addition, many negotiations seek trust as the objective and continue to build trust throughout the negotiation process. In the example of the negotiation with a hostage taker, the negotiator and hostage-taker may have not trust between each other at the very start.However, along the way, trust is built when both sides seek to understand the concessions that they can make, in order to gain what they want. While these initial impressions may also change as a negotiation unfolds, they create a powerful frame for interpreting the other party behavior and also provide a heuristic for duding negotiators' strategy choices (Lessons, Smith, 2012). As such, trust is important to keep a negotiation process going, and it is definitely an important factor to determine the relationship and outcome of the negotiation with the other party.As such, we cannot negotiate with people whom we do not trust. Earlier, contract binding was mentioned as a way where negotiation may occur even if there is no trust between two parties. Here, it is true that the two parties may have no personal trust in each other. However, according to Shapiro, even if there may be o personal trust, an institutional trust, also known as trust in a system, is what is present, leading to the creation of contract making (Shapiro et al. , 1992). With a trust in the legal enforcement system, it also cre ates a deterrence-based trust in order for a negotiation to proceed.Shapiro further added that this is a basic and ‘minimal condition' of trust in all negotiations (Shapiro et al. , 1992). This depends on the consistency in behavior and the threat of punishment if they do not maintain their promises to a certain commitment, and violate the documented agreements. In edition, it also depends on the gains for adhering to the agreements, such as rewards. Thus, the mentioned forms of trust will still be present even if we may have no personal trust.According to (Licked, 2000), even the most complex and sophisticated formal contract cannot stipulate every detail or possible contingency about the deal. Therefore, having personal trust in the other party is still critical. Without personal trust, a negotiation would not occur. This essay aims to discuss whether it is possible to negotiate with people whom we do not trust. Trust in this case, refers to having a positive expectation of the other arty and allowing ourselves to be dependent and vulnerable to the other party.There may seem to be cases where we may not have personal trust in a person and yet will still be able to negotiate. However, as mentioned in the essay, it may seem that trust is not present, but we may have a different form of trust, an institutionalized trust and most definitely, a deterrence based trust to ensure that we do not fall victim to breaches in agreements. The essay may show that it requires trust to form only cooperative agreements. However, likewise in competitive negotiations, it can still occur as deterrence based trust will be present to ensure hat agreements are not breached.As mentioned in the essay, without trust, we will only focus on our own objectives and interests and thus, will not allow the other party to gain anything. As a result, the negotiation will only come to an impasse if agreements cannot be made. In addition, without trust, we will be suspicious and skeptical of the information that is shared with us. This causes us to develop paranoid conditions and result in a conflict instead of being able to negotiate. To conclude, it is certainly critical to have some form of trust in a negotiation. However, it s also good to have relative trust, rather than an absolute trust.Having a fair share of distrust will definitely help us draw caution to what concessions we make. Ultimately, we cannot negotiate with people we do not trust at all. Trust will enable both parties to reach a solution that is needed or accepted by both sides, when we are not able to achieve them on our own.

Qualitative methods for social health research Essay

Qualitative methods for social health research - Essay Example Academic performance is the reason for studying to determine the level of intelligence. Motivation is a crucial aspect in validating the theme of academic performance. In the presence of adequate amount of motivation, there is a driving factor for academic performance. As responded by participant A, motivation will enable one to balance work and studies due to an impending aim of achieving results in both. When asked on How satisfied the participant is with academic achievement, participant A responds, that, â€Å"My study is going good beside my work time as I am a part time employee who is working only in the weekend and the rest of the weekdays I am a full time student so I manage my time to student during the weekdays†. While when asked on the circumstances under which he feels academic achievement is linked to work load, participant C stated that, â€Å"If u work less u will do better in your study as you will have more time to study and do the work on time as if you hav e part time work it will be easy than full time work and full time study, it easy to make you feel stress and scared†. In the assessment of the implication of work on academic performance, participant D was responded that â€Å"work does not affect my studies to a certain degree, because I have less time to study for my subjects†. Stress is a normal reaction to actual or potential stimuli. Basing on the theme of stress, most literature sources agree that stress is a normal response to a stimulant, which may be an actual or a potential threat. When one is faced with more than one task to perform in the study, work and life, stress ensues. However, as responded by participant B, a well balance of time within the daily activities helps in the management of stress. When asked how stress affects in relation to study, participant B says â€Å"stress affects my performance ability and decrease my productivity both at

Communication in Health & Social Care Essay Example | Topics and Well Written Essays - 1000 words

Communication in Health & Social Care - Essay Example This paper will look at communication in the health and social care area and the factor that affect communication in this sector. Factors that affect communication in health and social care area include beliefs and values. Service providers should embrace cultural diversity in order to be able to offer services. If the care giver is not aware of cultural diversity, it is impossible to communicate effectively. Care givers should be competent in the knowledge of culture. This is because people in every culture have their beliefs and values. In a place where there is multiculturalism it may be difficult to communicate (Dickson, Hargie & Morrow, 1996). This calls for care givers to be well informed when it comes to the culture of people they are serving. People perception and understanding of heath and heath care process is developed through their culture and social values. Peoples’ thinking on health and social care is based on their beliefs and values. In the United States health and social care providers are socialized into the culture of their profession. They are trained in the western scientific or bi omedical global view (Argyle, 1994). This teaches the professionals to hold and accept beliefs, norms, and values. This is good because the care givers who know their culture can also appreciate other people’s culture. The more a care giver knows of their culture the more they can accept the patients’ world view. Beliefs of people can affect their compliance with treatment, thus a health care giver should always be able to understand the beliefs of a patient for the service provided to be useful. The care giver should respect the patients’ beliefs and not dismiss their traditional practices. Some beliefs can make a patient lose trust on the care provider thus losing cooperation needed for successful healing and therapeutic relationship. For example, beliefs on herbal medicine may make a

Tuition assistance in the work place and its effects on retention Coursework

Tuition assistance in the work place and its effects on retention - Coursework Example The employers’ acts intends at enhancing employees’ loyalty and retention or longevity given that the employee continually expands their knowledge and skills while working. In my proposal of Tuition assistance in the work place and its effects on retention, I identified numerous factors that make tuition assistance programs by employers extremely effective. As an employer, ensure that there exists an educational assistance program in one organization, align the company goals and employees’ goals, define a strategic plan around the program, utilize online colleges and universities, and regularly keep track of success measurements for the tuition assistance program. Extremely effective tuition assistance could save a firm much money especially by regulating tuition to low cost college course providers and ensuring that all employees’ educational needs align with the company interests (Flaherty, 2007). Besides saving money, the firm could also reduce employees’ turnover rates given that educated employees better understand their responsibilities, have greater job satisfaction, and opt to remain with the company

Homework 5 Speech or Presentation Example | Topics and Well Written Essays - 500 words

Homework 5 - Speech or Presentation Example 2) Historically, the average amount of time to assemble an electronic component on a production line has been 14 minutes. The supervisor of this production line is interested in determining whether this is no longer true. Assume that assembly time is normally distributed with a known population standard deviation of 3.4 minutes. The supervisor times the assembly of 25 randomly chosen components, and finds that the average time to assemble is 12.6 minutes. Is there sufficient evidence to suggest that the population mean assembly time is not 14 minutes at the 1% level of significance? What is the p-value? 3) The manufacturer of a new chewing gum asserts that at least 80% of dentists prefer their type of gum. An independent consumer research firm decides to test their claim. The findings of a sample of 200 dentists indicate that 76% of respondents actually prefer the manufacturers gum. At the 5% level, is there sufficient evidence to suggest that the population proportion of dentists who prefer the manufacturers gum is less than 80%? What is the p-value? 4) A college professor is interested in determining the relationship between the number of hours a student sleeps prior to an exam and a students exam grade. The joint distribution is assumed bivariate normal. The professor draws a random sample of four students and records each students exam grade and hours of sleep preceding the exam. These data are found in the table below. The professor runs a simple regression, but has misplaced her regression output. Recognizing that Exam Grade is the dependent variable and Hours of Sleep is the sole independent variable, she asks you to perform the

Crepe in Japan Essay Example | Topics and Well Written Essays - 1000 words

Crepe in Japan - Essay Example Japanese crepes often offer several filling combinations, majority of them are sweet. Crepe business in japan needs many strategies to ensure that the business maintains its high standards in addition to retaining and attracting more customers. Japanese crepes are considered soft and crispy. They do not only serve as crepes for dessert but also crepes for savory that can be as a lunch meal. Crepes in japan do have several savory choices that make individual crave for it by just looking at it. Crepes In japan is almost reaching points of declining since less promotional activities are being used as strategies to enhance the sales. The consumer curiosity helps in enhancing the sales growth of crepe in japan. In japan, crepes products are directly distributed to the outlets to expand the markets. The crepes in japan come in various flavors including the crispy and savory crepes. Most of the crepes in japan are on the spot as desserts. Everything that entails the making of the crepes from the icing to the fruits, all are made on the same spot when an order is made. They are with no preservative being added on, and this offers an alternative to enjoy the dessert. The option of not using preservatives in the preparation of crepes in japan gives an alternative to the individuals who are not always seeking crispy crepes but also the yummiest at the same time. These desserts are one hundred percent made from the Japanese recipe that are original and thus attract several Japanese customers. Crepes in japan are sold just like any other foodstuffs; high quality and fresh. The outlets that serve to sell the crepes are made in a way that they look comfortable, cozy and have a unique atmosphere.

Technology in diplomacy Research Paper Example | Topics and Well Written Essays - 2000 words

Technology in diplomacy - Research Paper Example This has resulted in Austria shifting to paperless records, during late 1990s, while MFA of Latvia was awarded for the best web-site in 2003.(â€Å"Information and Communications Technology† 193) Foreign ministries around the world are still struggling to engage the benefits of information technology in their management of external affairs. However, some of the countries have realized the potential of using â€Å"Web 2.0†. A good example in this direction is Denmark, which has created a â€Å"virtual working group†. The function of the group is to arrange meetings of all members, from all corners of the world, using video-conferencing. This way the working group has better coordination; as it has members from all units, which are relevant to the working of country’s MFA, along with its branches in Africa, America, UN and EU countries. Utilizing the full potential of information communications technology (ICT), Denmark has identified the five principals of E-management, mainly applicable to a MFA manager. These include usage of intranet, on daily basis, for sharing ideas and communication. The concerned manager is required to make use of best practices, using IT tools, to set an example for the staff. In addition, the manager must ensure that the staff members are fully familiar with information technology, having the required skills. The manager is required, personally, to be fully conversant with the E-Government Strategy of MFA; while understanding its contents, perfectly well, which would help in contributing to the policy guidelines, in a positive manner. Canada, being the other country, which is utilizing the full potential of ICT, started an e-exercise, called ‘Public Diplomacy Network’, which involves offering of suggestions and other tips to the government on its foreign affairs policy. Accordingly, public awareness has been created among the people, through this network, regarding the government policies on inte rnational issues. Mexico is yet another country, which has growing network of foreign consular posts, in United States, sharing the best consular practices, while mutually learning, through the foreign ministry’s intranet. (â€Å"Information and Communications Technology† 194-5) With the rapid developments in the field of ICT, it is essential that all concerned keep pace with same, while understanding the changes, in a positive manner; which can help solving the problems, almost instantaneously. The social network sites, like face-book, have set an example for others, regarding best use of ICT. ICT applications After the use of computers as simple machines, their versatility increased with use of ‘local area network’ (LAN), which made inter-connections possible. Hence, many MFAs are using this technology even now, to stay connected. Then came the use of ‘wide area network’ (WAN), which meant keeping different ministries and departments of a co untry connected. Most European countries have adapted to this technology, in 1990s. However, many are still hesitating to use it, as they fair leakage of sensitive data, particularly pertaining to their MFAs. ‘Virtual private network’, or intranet, has been used to cover all the embassies of a country, as it widens the scope of WAN. However, many smaller countries are not able to go for such technology, due cost and skilled manpower constrains. Countries like China, Japan and India have

Women's Labor Project Coursework Example | Topics and Well Written Essays - 250 words

Women's Labor Project - Coursework Example I have secured a new job in an apparel factory, many of the women that I work with are also from Italy so we get the opportunity to swap stories in our mother language. It is difficult to communicate with some of the other women because they often speak different languages and it is sometimes difficult to communicate in English. Nevertheless I have become good friends with a German lady who speaks English quite well, and even some Italian. It was not always easy for Aronne and I in our new life. Upon our arrival we had to live in a settlement house in the city and it was difficult for us to find good jobs. There was a lot of crime, in this new apartment complex but we life next to some good Italian people and we look out for each other. In the market it is easy to find some of the foods that we are accustomed to but there are a lot of people from very exotic places and it is always exciting to explore new areas. Recently some of the people at work asked me to join a union. I am reluc tant to come on board simply because the management is so strongly anti-union that I would fear for my job security and possibly even for my health and safety.

CIA Operation PBSUCCESS Essay Example | Topics and Well Written Essays - 1000 words

CIA Operation PBSUCCESS - Essay Example "PB" here stands for "Presidential Board" while SUCCESS is used to express the general optimism and confidence that this operation would achieve its objectives. Its main objective was overthrowing the popularly-elected Guatemalan President, Jacobo Arbenz Guzman. This was because his government had presented various new policies that the US was of the opinion that they were Communist. The US suspected the influence of the Soviet Union in this. The US clearly hated Communism and all its principles. They could therefore do anything to stop it. Moreover, Communism threatened to oust the US as the world's super power. They had to fight this at all costs to continue with this domination. For Guatemala, Communism posed a threat to the US interests in that country and even neighboring ones. They had to intervene to secure these interests. The US was therefore killing two birds with one stone in Guatemala. The US feared that Guatemala becoming a "Soviet beachhead." Guzman introduced land reform acts were seen as antagonizing United Fruit Company, a US-based multinational. This company had great stakes in the earlier Guatemala administration and urged the US to intervene. The operation took place between 1953 and 1954. ... Later there was another operation known as "Operation PBHISTORY". Its aim was gathering and analyzing Guatemalan government documents. The US would later use these documents to portray Guzman as puppet of the Soviet Communists. During the administration of two of Guzman's predecessors, Jorge Ubico and Manuel Cabrera, Guatemala was open to foreign investment, with Ubico specially favoring the United Fruit Company. The UFC then invested heavily in the country. It bought controlling stocks of the rail, electricity and telegraph utilities. It won control over most of Guatemala's best land. Ubico administration greatly protected the interests of the United Fruit Company. Soon after the inauguration of Dwight Eisenhower as the US President in 1953, scrutiny of CIA covert action operations started. Eisenhower set up an evaluation known as Solarium that comprised three committees that did an analysis to find out which action plans should go on. Later, CIA's covert activities were kept under close check. Questions arose as to whether CIA covert operations were proper and if they served national interests (Cullather, 2006). According to David Bruce, a presidential advisor on foreign intelligence activities, these covert actions were responsible for sparking turmoil and raising doubts about the US in numerous countries worldwide. The US faced political condemnation worldwide because of the role it played in having President Guzman overthrown. The US damaged its reputation in Latin America and also angered its allies. It was heavily criticized even by the United Nations (Kinzer and Schlesinger, 1999). There was strong resentment in the Caribbeans and Latin America. This played a part in damaging the stability of Guatemala and Latin

Correctional system Essay Example for Free

Correctional system Essay Does the conservative approach or liberal approach improve the correctional system. Please explain and give an example Though conservative approach has been criticized greatly over its dominance and operation in the last two decades, it still forms the best approach for crime control and correctional consideration. Over the last two decades, the conservative zero tolerance to crime has achieved a lot by segregating the criminals and correcting them in isolation. According to the pattern theory, it is clear that once a criminal has been established, it is very hard to prevent latter crime. Notably, keeping the criminals in prisons makes it possible for the administration to mobilize resources towards their correction with ease. To add to that, it is much easier to monitor the criminals reform progress. According to the Oklahoma Board of Corrections, John Lilly Correction Centers criminals are able to refocus on their actions and therefore make lifetime decisions to change. In addition, the desire for revenge by the offended reduces greatly when the individuals who committed crime are away from the community. Justice entails holistic evaluation of the scenario and the general re-evaluation that the offender gets due to punishment from his/her actions. According to criminal analysts, the presence of an offender (like a rapist) can hamper the psychological recovery of the offended. Though this system has been highly effective amidst vast criticism, it has the following disadvantages which should be addressed to perfect it. With the criminal correction system taking place at segregated places, there are high chances of minor offenders mixing with hard criminals which can make the formers to assimilate the traits of latter. Therefore, they should be separated and greater surveillance offered to ensure compliance. To add to that, the approach requires vast resources to maintain and effect the correctional measures to the vast prisoners in the US jails. To borrow from the liberals, it would be important to ensure an outward outlook of the correctional approach as a mode of preparing the inmates to get back to the community after the correctional period (Byrne, 2005). 2: Does the restorative justice approach improve the criminal justice system. Please explain and give an example Recently, criminal justice has taken different considerations that indicate possible future changes in the overall application of the justice system. Restorative justice is a broad term which refers to institutionalization of peaceful approaches to addressing harm, problem-solving, and violations. This system has assisted improved criminal justice greatly by bringing the two parties (offender and the offended) to the same table for reconciliation. Under this system the offender seeks reparation directly or indirectly from the offended. Restorative justice assists in addressing all the underlying issues that hold the offended captive until justice is done. During the Truth and Reconciliation Commission of South Africa after the oppressive regime of apartheid, it was possible for the offended to totally bring out their anger, grief, pain, and sorrow they encountered during apartheid. As indicated by Piagets theory of personal development, what is in ones mind persists for long until such a time that the correct platform for expression is established. Restorative justice therefore gives people the correct chances to forgive one another totally. As indicated by the South Africa Truth and Reconciliation commission, it becomes much easier to develop better relationship between the offender and the offended after the process. In adversarial legal process and civil litigation processes, lawyers simplify reduce the issues between the offenders and the offended to only legally relevant considerations to protect their clients. However, restorative justice is better in that all aspects are addressed holistically and rehabilitation exclusively undertaken to enhance later coexistence and development. Restorative justice therefore assists in achieving the correct balance between the rights of the offenders and the needs of the victims. Besides, it strikes the balance between the need for rehabilitation of the offenders and the main duty to protect the public from such criminals. This system should therefore be encouraged due to its conterminously acceptable ideals at all levels (Jaimie, 2005). Q3: Does community-based correctional options address crime control? Please explain and give an example. Arguably, community based correction options assist in reducing crime in the society by ensuring that the criminals are reformed in a model that prepares them to be assimilated back to the society. Whereas other correction systems emphasis on the need to harshly treat the offenders for them to realize their mistakes and reform with time, this method is considered to be a softer option as it is based in the community. To add to that, it gives more focus to developmental skills attainment that assist offenders prepare for productive lives after their correction time is over. Oriana House is a community based correction unit that operates in Ohio and Akron. In this CBFS, felony offenders are sentenced to undergo substance abuse treatment, job training, educational services, and cognitive skills that can assist them after completing the community service. The offenders usually get time to mix with the community in various activities and educational programs. This system redirects the focus of an individual to constructive thinking as opposed to destructive format that is achieved by the conservative approach. However, it has been criticized for failing to ensure that strict measures are taken by the corrective system (Curt Anne, 2008). About 5% of the people under the system are likely to recidivate back. Reference list Byrne, J. M. (2005). Crime Control Strategies and Community Change-Reforming The Surveillance vs Treatment Debate. Lowell: University of Massachusetts. Curt, R. Anne, B. (2008). Introduction to Forensic Psychology: Research and Application. New York: Sage. Jaimie, B. (2005). ‘Restoration or Renovation: Evaluating Restorative Justice Outcomes’. 12(1) Psychiatry, Psychology and Law 12(1):185-200

Separation of Histone Protein

Separation of Histone Protein For estimating protein mixture qualitatively most widely used method is SDS-Polyacrylamide gel electrophoresis (SDS-PAGE). According to size of the protein, this SDS-PAGE is separate the protein and purification of protein is to be monitored by this method, and relative molecular mass of protein can also be determined. In this SDS-PAGE anionic detergent is SDS. Before loading the sample, the samples are boiled for 5minutes, that contain s SDS and à ¯Ã‚ Ã‚ ¢Ãƒ ¯Ã¢â€š ¬Ã‚ ­mercaptoethanol in the buffer. While boiling the sample the SDS act to denature the protein and where à ¯Ã‚ Ã‚ ¢Ãƒ ¯Ã¢â€š ¬Ã‚ ­mercaptoethanol decrease the disulphide bridges of the protein that are holding tertiary structure of protein .by this denature process the protein get fully denatured and form a rod shape structure with negatively charged molecules of SDS throughout polypeptide chain. Every couple of amino acids binds with one SDS molecule on average. Due to the negatively charge SDS the structure remain s as rod like. So repulsion take place between the negatively charge on proteins and no folding occurs and remains rod shape. In the sample loading buffer, contains bromophenol blue and sucrose or glycerol. The bromophenol blue is helpful in monitoring the sample, when electrophoresis running and glycerol give density to the sample that can settle at the bottom of the well on stacking gel. The samples are loaded on the electrophoresis gel, which is made up of two gels .the lower gel is main separating gel and upper gel is stacking gel. This stacking gel helps in loading the sample into wells and had large pore size. Where protein sample moves freely and makes the protein sample concentrate and forms sharp band and enters into main separating gel with effect of electric field. Here isotachophoresis take place. The glycinate ion which is negatively charge has lower mobility than SDS-proteins molecule in running buffer than cl- ion in stacking and loading buffer. At the higher field strength both cl- and glycinate travel at same speed. So these ions and protein adjust those concentrations. The separating gel has higher PH environment, once glycine receives it become highly ionised state and mobility increases. By this the cl- and glycinate leaves the SDS-protein molecule. Now the SDS-Protein molecule moves towards the anode in separating gel by the effect of electric field. Here the protein having smaller size moves faster and reaches to the bottom of the gel than protein having larger size, with the help of bromophenol blue dye we can indicate the electrophoresis front because smaller particle unretarded the dye colour. When dye comes bottom of the gel then turned off the current, remove the gel from the sandwich properly and stained with coomassie brilliant blue and then by using destainin g solution, gel is washed. Depending on the protein size the preparation of polyacrylamide gel is used like 15%, 10% and 7.5%. By the help of the standard protein the mobility of unknown can be calculated by using calibration curve. In SDS-PAGE the protein should give single band, then that protein is said to be pure. So for purification protein process SDS -PAGE is most widely used. To the cluster of eight histone protein (H1-H8) DNA is wounded around. By the help of histone and DNA chromatin is made. The regulation of expression of genes and organisation of DNA is done by the help of histone proteins. Due to histone protein modification we can keep the genes active or silent and modifications are like methylation and acetylation. The transcription factors take place by the modulate accessibility of DNA by histone modification. DNA access might blocked by histone methylation to transcription factors. Electrostatic interaction might change due to histone acetylation in chromatin and allows transcription after opening up DNA. In blood cells development in chicken the principle of the histone modification is clearly demonstrated. In the transition the structural and functional role is played by histone protein between the states of active and inactive chromatin.high degree of conservation consists in histone . This is due to structural maintained constraining the e ntire nucleosomal octameric core. In the gene regulation and epigenetic silencing the diverse role play by a histone proteins.DNA replication, repair, transcription and recombination are influenced by the post translational modification, interactions with chromatin remodelling complexes and histone variants. DNA is packed in the nucleus and forms a complex called chromatin. The first level of chromatin organization is represented by the nucleosome core particles. The octameric core is composed of 146-147 bp of DNA that are tightly wrapped around two copies of histone H2A, H 2B,H3 and H4. Nucleosome cores are associated with linker histone H1 and separated by variable length of linker DNA. Core histone internucleosomal interactions are mediates by composing packed nucleosome arrays to start helical model. Due to the presence of histone fold domain the core histone are characterised and variable lengths of N-TerminaL tails are extensive subjects for post translational modifications. T he epigenome are the component of post translational modifications hence that includes protein connected to its gene and changes in DNA occur. For regulation of gene expression the epigenetic mmodifications are act as switches. DNA and histones are its chemical modifications .which does not disturb the sequence changes to DNA. The organisms reveal a variety of striking similarities despite histone tail and core variation due to characterization of structural nucleosome core particles. Using structural information they reanalysed histone fold domain variably sequence in a novel fashion. The variable pair of histone protein are H2A and H 2B and the conserved one are H4 and H3. In eukaryotes histone proteins are associated with DNA and are positively charged, this is due to presence of positively charged amino acids like lysine and arginine . H 1, H2A, H 2B histone are rich in lysine and H3 , H4 are rich in arginine. Each nucleosome consists of 8 histone proteins. Around one nucleosome to another nucleosome 200bp is present in DNA. In a circle of 1 nucleosome 146 bp are present. Where 54 BP are present in connection link of DNA between 1 nucleosome to another nucleosome. In nucleosome H1 histone is absent.here linker DNA connects two nucleosomes and H1 protein present in linker DNA. H1 protein takes an active role in formation of eukaryotes and heterochromatin. Genetic and epigenetic changes both involved in breast carcinogenesis and it is a multi step process. Epigenetic is a change that observed in gene expression in both reversible and heritable by the gene sequence without alteration. In cancer that influence the two major epigenetic changes are DNA methylation and histone modification interactions is well orchestrated. Malignant and premalignant breast neoplasm is methylated by involvement of several genes in metastasis, proliferation and antiapoptosis. In breast cancer treatment with other systemic therapies, histone deacetylase inhibitors become synergistically an important class of drugs. Potentially reversible processes are epigenetic changes and for finding novel therapies and refined diagnostic of breast cancer many efforts has been done for understanding the mechanism. MATERIALS AND METHOD: 30% W/V Acryl amide /Bis acrylamide Tris Hcl 3.0M, PH = 8.8 (lower gel) Tris Hcl 0.5M, PH =6.8 (upper gel) Bio-rad mini protean tank TEMED Ammonium persulphate (APS 25%W/V) Running buffer Bromophenol blue Sample buffer Coomassie blue stain Human recombinant proteins H4,H3.3, H2B, H2A EXPERIMENTAL PROCEDURE: SDS -PAGE GEL PREPARATION: PREPARATION OF GEL CASSETTE SANDWICH: The casting frame is taken and place on the flat surface. Select the glass plates to make a sandwich and place the short plate on the spacer plate and fix the casting frame to make sandwich. Fix the casting frame to the stand and the sandwich glass plates on the gray rubber gasket. Then checked the sandwich plates with distilled water to ensure any leakage occur. Prepare the resolving gel into a beaker without adding TEMED and APS. Add TEMED and APS into the prepared resolving gel and mix the solution homogenously and immediately pour the mixed solution into the sandwich plates, more than half of the glass plates. Allow the resolving gel for 35-40 minutes to get gel polymerised. Wash the resolving gel with distilled water and discard the water from sandwich, dry the inner surface by using filter paper. Prepare the stacking gel into another beaker without adding the TEMED and APS. Added TEMED and APS and mix equally and pour it on the top of the resolving gel and gently place the comb on the top of the stacking gel. Then leave the stacking gel overnight for its polymerization. RESOLVING GEL AND STACKING GEL PREPARATION: Resolving gel: acrylamide/bis-acrylamide 10.0ml,3.0M Tris /Hcl (PH=8.8) 3.75ml,dH20 15.8,10% SDS 0.3ml,TEMED 0.015, Ammonium Per sulphate 0.15. Stacking Gel: Acrylamide/bis acrylamide 2.5ml,0.5M Tris /Hcl (PH 6.8) 5.0ml,dH20 12.26ml,10% SDS 0.2ml,TEMED 0.015ml,Ammonium persulphate 0.04ml. Separation of H2A/H2B/H3.3/H4 Human Recombinant Protein using 1D SDS-PAGE Gel . After overnight polymerisation taken out the comb carefully and well are washed with running buffer. Remove the gel sandwich from the casting stand and allow to place them in the electrophoresis tank placing short plate facing inwards. Fill the gel electrophoresis tank with running buffer up to halfway between inner chamber i.e. 125ml and in the mini tank add 200ml of running buffer. SAMPLE PREPARATION AND LOADING: Taken the sample of histone protein of à ¯Ã¢â€š ¬Ã‚ ±Ãƒ ¯Ã¢â€š ¬Ã‚  Ãƒ ¯Ã‚ Ã‚ ­l and added into the sample buffer of 20à ¯Ã‚ Ã‚ ­l eppendorf tube. The protein samples are labelled to each tube. The histone protein samples are heated to 100o c for 2 minutes in hot block and at room temperature allow cooling down. Now samples of histone proteins are allowed to load into the well of 20à ¯Ã¢â€š ¬Ã‚  Ãƒ ¯Ã‚ Ã‚ ­l of each sample with the help of loading gel tips and while loading, load the sample carefully and slowly without air bubbles and allow the sample to settle down at the bottom of the wells. Taken molecular marker of 2à ¯Ã¢â€š ¬Ã‚  Ãƒ ¯Ã‚ Ã‚ ­l and loaded in another well for the identification of the proteins migration. GEL ELECTROPHORESIS : Cover the mini tank with lid properly by using colour code present on the banana plugs. Connect the gel electrophoresis tank to power supply by using 200volts of constant current for about 35-40 minutes until samples runs more than 3/4th of the gel. Stain and de-stain gels: After reaching the sample nearly bottom of the gel turn of the power supply to the gel electrophoresis tank and disconnect the electric leads. Discard the running buffer to avoid splitting and carefully remove the gel sandwich, gently separate the gel from plate by using sharp wedge, separated gel is placed in coomassie blue stain solution of 20-30 ml for 30 minutes on shaker for constant shaking. After the 30 minutes discard the stain solution and wash the gel with distilled water for 4- 5 times for constant time intervals and incubate at room temperature for overnight by placing on shaker. Finally rinse the detained gel with distilled water till the protein bands can clearly visible. Taken the pictures by using camera. RESULTS: By observing the obtained result after running the histone samples H4,H3.3,H2B,H2A in 1D SDS-PAGE . The separation of samples has been seen on the gel by using a dye coomassie blue stain solution. While observing the samples are run on gel according to their molecular mass and get separated from each other. The sample histone protein H4 shows little bit difference in observation which is present near to bottom of the gel ,that shows it run little bit faster than other histone samples due to its smaller size. While observing the other histone samples like H3.3,H2B,H2A they has no lot of difference in separation to differentiate from each other. DISCUSSION: The present experiment explains the isolation of human recombinant protein H4, H3.3, H2B and H2A by using the 1D SDS-PAGE. By observing the obtained result that found the histone protein H4 migrated little bit faster than the other samples .where as other histone samples H3.3, H2B, and H2A are observed ,there is no lot of difference in the migration to differentiate from each other. According to Kornberg, R.D when they performed the experiment on histone protein of human recombinant, found the histone protein H4 migrate faster than other protein and appears to be at 11 kDl . Where H3.3 appears near 15kDl,H2B appears near 14 kDl ,H2A appears near 12 kDl with these result we expect to be the same result but according to the above result that H3.3 ,H2B,H2A does not show much separation in migration of protein sample. So for getting such result may have many reasons that might have not loaded the samples with equal volume or properly loaded in the wells or power supply to the electrophor esis tank is not adjust properly or one sample over float into other wells while loading. The separation of the histone proteins that observed by different authors are histone proteins which undergoes non-acetylase that migrates faster than the protein that undergoes monoacetylation and acetylated derivatives. In this sequence the histone proteins are clearly separated in the core histone protein by using 1D SDS-PAGE. The retarded mobility are shown when the histone protein is highly acetylated compared with non acetylated parent compared. The variants are observed in histone protein due to differing of amino acids in the sequence. Histone proteins undergoes different biological conditions and form to be post synthetically modified like ADP-ribosylated, phosphorylated and acetylated .by using SDS-PAGE the purity of isolated proteins are identified. In the present experiment if the mixture of four histone proteins would need to be separated by the same technique. I would preferably take the three consequent results of same and expected as follows molecular size of H4 has l ess kDl than H2A, H2B and H3.3 in kDl. Few journals and reviews found to be support my hypothesis like Kornberg, R.D(1977) and Herbert and Linder(1992).

Tetanus Toxin: Structure and Purification

Tetanus Toxin: Structure and Purification Tetanus is regarded amongst the most severe and fatal disease since ancient times [1]. Tetanus is termed from a Greek word ‘Tetanos’ which means- to contract *. The disease is generally initiated due to deep wounds, cuts, and during catastrophic situations like natural calamities, physical trauma, etc. [a] It was first discovered by Hippocrates in early 19th century *. However it was etiologically described by Carle and Rattone who were first to produce tetanus in animals by injecting pus extracted from an infected human with the same disease in 1884. During this same period Nicolaier also produced tetanus in animals from soil samples. Further research in 1889 by Kitasato revealed that animals were infected by this disease when they were injected with a particular organism isolated from a human patient *. Nocard in 1897 revealed that this disease can be treated by the use of its antitoxin. Moreover in 1924 the significance of toxoid came into existence during World War I which was formulated by Descombey and this passive immunization against tetanus was majorly used during World War II *. Structure of tetanus toxin: The tetanus toxin is of 150kD comprising of three fragments i.e. A,B and C having a molecular weight of 50kD each [n]. Fragments A and B were observed to be non-spastically toxic and also to block the release of catecholamine. It also inhibits the action of synaptic nerves and thus exhibits an important role in the toxicity of the toxin. On the other hand fragment C is regarded as the non-toxic subunit but retaining the required antigenic properties of the toxin. This fragment is seen to bind with gangliosides, motor endplates and synaptic membranes. It also helps in transportation of the toxin from the periphery to the central nervous system [o]. Krieglestein et al. in 1990 stated that tetanus toxin is a 151-kD protein. The complete amino acid sequence is known. The mature toxin is made of two peptide and contains 10 half-cystine residues. Treatment with 4-vinylpyridine in the presence of 6M guanidine converted six of them into s-pyridylethyl cysteine residues are determines by amino acid analysis. When alkylation was preceded by mercaptolysis, all 10 halfcystine residues were recovered in the s-pyridylethylated form. It was therefore concluded that the toxin contains six sulfhydryl groups and two disulfide bond [r] Mode of Action: Rossetto et al. in 2001 reported that the neuroparalytic syndromes of tetanus is caused by neurotoxins produced by bacteria of the genus Clostridium of 150 kDa proteins consisting of three-domains, endowed with different functions: neurospecific binding, membrane translocation and specific proteolysis of three key components of the neuroexocytosis apparatus. After binding to the presynaptic membrane of motoneurons, tetanus neurotoxin (TeNT) is internalized and transported retroaxonally to the spinal cord, where it blocks neurotransmitter release from spinal inhibitory interneurons. TeNT cleave specifically at single but different peptide bonds, VAMP/synaptobrevin, a membrane protein of small synaptic vesicles [s]. Kegel et al. in 2002 stated that the 50kD ligh chain subunit comprises of zinc metalloproteases which cleaves synatobrevin that is not involved in neuroexocytosis [t]. Foster in 2009 Stated that TeNT enters the body via wounds and initially binds and internalizes into the peripheral terminals of motorneurons where it is transported by retrograde axonal transport to the motorneuron in the spinal cord. TeNT is transported to somatodendritic postsynaptic sites and is released into the synaptic cleft where it undergoes receptor mediated uptake into the presynaptic termini of the inhibitory interneurons, from where it translocates into the cytosol and inhibits neurotransmitter release. [u]. Starting material for purification of tetanus toxin: Raynaud in 1951 developed a technique of using non-autolyzed toxin direct from the organism i.e. Clostridium tetani [i] .This technique gave an advantage of obtaining a more concentrated form of toxin as compared to that obtained from the filtrates [i][j]. For this purpose the organism was generally cultured and subcultured using Tarozzi medium* and modified Tarozzi medium [j] Latham medium was also widely used for the same reason [j] [k]. M. Matsuda et.al in 1989 also used modified Latham medium for culturing of Clostridium tetani [o]. Muller and Miller in 1954 investigated that pancreatic digest of casein contained some inhibitory content which was solved by charcoal treatment [y]. Toxin was also extracted by treating the bacterial cells in hypertonic solution using 0.1M sodium citrate and 1M sodium chloride as stated by Bernard Bizzini et.al [q] Conventional method to produce tetanus vaccine: The Harvard strain of Clostridium tetani is grown in a fermentor for about a week using a semisynthetic medium. This leads the bacteria to lyze and release the toxin obtained in the supernatant. This method yielded about 60-80 Lf/ml. This yield is then filtered and detoxified using formaldehyde. This reacts with the toxin molecule mainly the amino groups of lysineresulting in imine formation, further reacts with the unstable groups of imidazole or phenol ring finally involves a cross-linking reaction between the both the amino groups. Formaldehyde also affects the 3-D structure, therefore making the toxic conformational epitopes [y]. Purification by HPLC: Kunihiro Ozutsumiet.al. in 1985 used extracts from the organism for purification of tetanus toxin using High performance liquid chromatographic methods (HPLC) [j]. The toxin extracted from the previously described method was initially purified using ammonium sulfate precipitation followed by ultracentrifugation in order to get rid of the unwanted particulate matter by filtering it through a 0.2 um membrane filter. The concentrated sample in the equilibrating buffer at a pH of 7.5 proceeded through a final step of purification by running it on HPLC using a column of a TSK G3000 SW of 0.75 x 60 dimensions. This column was equilibrated using 0.1M sodium-phosphate buffer at a pH of 6.8 and the flow rate was maintained at 0.6 ml/min. The fractions obtained were tested for its protein content at 280nm using a UV spectrophotometer [j]. Further the efficiency of HPLC was compared with another gel filtration method using Ultrogel column [j] [o]. Purification using Sephadex G-100: For large scale production of tetanus toxoid, Alcohol precipitation was used for immunization purpose by Pillemer L. et.al [b]. However, Levine et.al in 1951 used to purify the tetanus toxoid by ammonium sulfate precipitation [c]. Later, further purification and characterization of the toxoid was achieved by filtering it through Sephadex gels using G-100 columns as stated by Williams C. et.al in 1965 [d]. This simplified and low cost method yielded four separable fractions of the toxoid where the first two fractions of 55-65% non dialyzable nitrogen possessed significant antigenic properties. The next fraction obtained was of smaller molecular weight and showed poor antigenecity when injected in animals; however the fourth fraction obtained was not identified but was predicted to be metabolic by-products of the organism and had no significant role [d]. Before running on the column the protein concentration was determined using a UV spectrometer at 280nm. Chromatographic gel filtratio n was performed using a column of 1.2 x 0.062 m dimension. The column was packed and equilibrated with 0.1M phosphate buffer with a pH of 8.5, additionally 1% formaldehyde can be added to inhibit the bacterial growth. The void volume after equilibration was maintained at 800ml at the flow rate was fixed at 80ml/hr. The sample loading volume was around 50ml and was concentrated to about 100,000 Lf. The four fractions were collected and were further seperated by recycling them on the same column [d]. This method gave an efficient insight on how to purify and separate different components of the toxoid. M Matsuda in 1989 carried out the separation of fragment A-B treated with urea by running it on a ccolumn packed with Sephadex G-25, equilibrated with 0.02M tris-HCL buffer containing trace amounts of dithiothreitol and urea [o]. Other gels such as Sepharose 4B and Sephadex G-200 was also used by researchers like Bernard Bizzini, Immunodiffusion test was also carried out using Ouchterlony’s method [o] [p] [q]. Papain Digestion of Tetanus toxin: Further research by Helting and Zwister in 1974 made possible to obtain fragment C from Tetanus toxin which has significant antigenic properties but lack pathogenecity and thus occupies a major role in immunization [e]. Helting et.al stated that Tetanus toxin can be degraded in a specific pattern. The mild papain digestion cleaves the F(ab) region. The papain enzyme breaks the 150kD toxin into two parts, one comprising of the C-terminal of the heavy chain i.e. of 47kD which corresponds to the Fragment C of the toxin whereas the other part of 95kD consists of N-terminal heavy chain subunit along with the lighter chain polypeptide forming the fragment B (refer to Figure 2). This Fragment B was observed to have a toxic effect on mice when injected with a sufficient dose and also has an adverse effect on the nervous system, thus it was necessary to purify and obtain only Fragment C for immunization and to further study its immune response [f]. The purified Fragment C was separated and ob tained by chromatographic methods and by using anti-Fragment C IgG [f]. Ulrich Weller in 1989 performed papain digestion for 16 hours of overnight stirring of the toxin at 25Â °C at a concentration of 40ug/ml. The toxin was suspended in 10mM sodium-phosphate buffer at pH of 6.5 with 1mM EDTA and NaN3 and 10mM cysteine. After the incubation period 0.5mM of Ll-chloro-3-tosylamido-7-amino-2-heptanone was added as a stop solution in order to inactivate papain by further incubating it at room temperature for 30 min and was then cooled to 0Â °C with saturated ammonium sulfate solution at pH 6.5 with further centrifugation. The precipitate was resuspended in the same buffer mentioned. This further proceeded for its separation and purification on Sephadex G-100 column and the fractions were collected at the flow rate of 15ml/hr b*. These fractions were further pooled and contrated using a Centiprep 10 concentrator and the buffer was changed to 0.5 M NaCl with 30mM Tris-HCl at pH 7.5. The fragments B and C showed up homogenously on SDS-PAGE. The fragment C was further dialyzed against 10mM sodium phosphate buffer at a pH of 7.5. The samples obtained were further checked for its protein content at 285nm and was determined by modified lowry method after trichloroacetic acid precipitation. They also ran an SDS-PAGE using rerducing and non-reducing gels and was stained by Coomassie blue-250 and the chains and fragments of the toxin were determined according to their known amino acid sequence b* Other methods developed to obtain fragment C: Fishman et al. (1992) Pointed out that the non-toxic binding fragment of tetanus toxin (fragment C) binds avidly to neural tissue and has a growing number of neurobiological uses. Its current utility is limited by both its high commercial cost and the complex procedure for its preparation requiring highly purified tetanus toxin. A short procedure was developed which prepares fragments of tetanus toxin from crude C. tetani extracts. The resultant proteins are atoxic with molecular sizes and immunological properties closely resembling fragment C. These proteins undergo retrograde axonal and apparent transneuronal transport in a fashion similar to fragment C [v]. Ledoux et al. in 1994 Indicated that tetanus toxin once internalized via receptor-mediated endocytosis, form membrane channels in order to traverse the endosomal membrane and enter the cytoplasm of the nerve terminal forming an association between neurotoxin monomers which results in an oligomeric form of the neurotoxin necessary for assembly of a channel through the hydrophobic interior of the endosomal membrane, thereby allowing passage of the neurotoxin or its active fragment through the resulting pore [w]. Technique used to test the specificity of the heavy and light chain subunits: Matsuda and Yoneda in 1975 isolated the heavy and light chain subunits from a toxin reduced by treatment with dithiothreitol-urea[g] [h]. Kunihiro Ozutsumiet.al. in 1985 used the technique of electrophoresis using sodium-dodecyl-sulphate polyacrylamide gel i.e. SDS-PAGE as shown in Figure (3). and was further used to put up a western blot in order to check the specificity of the isolated subunits obtained [l] [m] [j]. SDS-PAGE allowed the toxin to stack at 49kD corresponding to the fragment C subunit and 85kD comprising of the 4heavy chain subunit [j] Goretzki and Habermann in 1985 characterized enzymatic fragments of tetanus toxin by immunoblotting using a set of previously characterized antibodies and a set of novel antibodies. The selected antibodies recognized the light chain, fragment C (ÃŽ ²1) and the complementary piece (ÃŽ ²2) of the heavy chain when blotted on nitrocellulose. All toxin preparations contained intrinsic esteroprotease activity which became manifest in the presence of urea. The main product of papain hydrolysis is fragment C, which appears as a double band under non reducing conditions but is homogeneous when reduced. Chymotryptic digestion hydrolyses the heavy chain well but leaves the light chain largely intact. Tetanus toxin is very resistant against trypsin as compared with other proteases, although this enzyme splits numerous different links [x].

The Rotunda of the 1873 Vienna International Exhibition :: Architecture History

The Rotunda of the 1873 Vienna International Exhibition Austria hosted a world's fair in 1873, as it aimed to present itself as a world leader and the equal of England and France. The structure that served as the focal point and ideological locus for the Vienna Exhibition was its striking Rotunda, a feat of engineering and design, which is pictured during the fair's opening ceremonies on May 1, 1873. On that day, the streets of the Ringstrasse were wet with a cold drizzle. More than twenty thousand Viennese citizens came out to see the festivities, yet the area around the Rotunda, Leopoldstadt and Praterstern, did not seem even half full. Emperor Francis Joseph appeared at noon, signaling the beginning of the ceremonies. The program was unusually brief; it concluded in less than thirty minutes and lacked any type of formal speech. However, the one characteristic that immortalized that day was the music. The strains of the Imperial Opera's orchestra and the voices of two Viennese Glee Clubs united in performing Handel's March, the waltzes of Strauss, and the Austrian national hymn. While the music emerged as a high point of the initial celebrations, the atmosphere in Vienna during the fair seemed rather half-hearted. For the past several years, the nation had been enjoying a period of economic growth and domestic prosperity. However, just a fortnight after these opening ceremonies, the Austrian stock market underwent a severe crash, wreaking havoc on the domestic economy. This frustrated the efforts of the Austrians to convey a strong impression to the international community. Ironically, it also seemed that while other countries experienced success in selling their national products to the fair visitors, the Austrian displays lay untouched. For these reasons, the atmosphere in Vienna during the fair was not as jubilant as had been seen at other exhibitions. However, the crash occurred after the Rotunda was built, so the budget for the construction of the Rotunda strikes one as fairly impressive. In July 1871, the original amount allocated for its building was 6,000,000 florins. However, in September of 1872, an additional 6,000,000 florins was added. The website image is an 8 X 11 inch black and white engraving that appeared in the periodical Kunst und Kunstgeverbe auf der Werner Weltasstelling. One can note the presence of a small crowd, in their finest attire inside the building. It seems logical that this picture was sketched by an artist who had been present during the ceremony.

Effective Meetings - Essential to Any Organization Essay -- Business M

While effective meetings are essential to any organization and to getting work done, most of the meetings I attend leave me looking for a decision. These meetings also leave me tired and somewhat disillusioned on the vision of the company. I think a good meeting is not dissimilar to a football teams huddle. It should bring people together, facilitate decision making, assist people in taking responsibility, energize the participants, and contribute to building team effort within the organization. From attending and running more than my share of meetings I feel successful meetings are ones where attention is paid to three areas; content, design and process. Selection of content is crucial. At a meeting I recently attended we discussed issue...

Freud’s Theory of Psychosexual Development Essay

Sigmund Freud, born in 1856 was an Austrian neurologist who would later go on to found the discipline of psychoanalysis. He is best known for his theories of the unconscious mind and repression and his concept of the dynamic unconscious suggesting that it is our unconscious mind that determines how we as individuals behave, Freud also believed that the unconscious mind established sexual drives as the dominant motivation of human life. He considered the unconscious mind as being the source of mental energy which determined behaviour, basing his findings on the results of his use of hypnosis where he found that he was able to produce and remove symptoms of hysteria. There have been numerous approaches in the field of psychology that have put forward the belief that behaviour is directed by an individual’s goals but the idea behind a goal-directed unconscious is an original Freudian concept. The main underlying belief of this theory is that any individuals’ behaviour is the direct result of the influences that prior experiences have had on them where these influences have an even greater effect if they are from our childhood. Freud believed that our early experiences formed the solid foundations on which we would build the structure of our life and that the adult personality is indeed formed in childhood according to the situations, treatment and feelings experienced as a child. Freud defined the human psyche as comprising of three parts, the unconscious or sub-conscious containing material that we are unable to bring into our conscious awareness and therefore unknowable. The preconscious which consists of information that is not at the present moment in our conscious awareness but is stored in our memory and can if need be easily recalled to the conscious level; and the conscious part of our mind which is where all current and new incoming content is processed. Within these parts operate the Id, the Ego and the Super Ego that work together to create complex human behaviours. The Id is the only part of our personality that is present from birth and is entirely unconscious, seeking instant gratification and fulfilling instinctive human needs. The Id is governed by the pleasure principle desiring the fulfilment of all desires, needs and wants. If these are not immediately satisfied this results in a state of anxiety or tension. The Id serves of great importance early on in life, a child will cry as a  result of their Id if they are hungry or in discomfort and ensures that their needs are met. Later on in life it is not always realistic or indeed possible to immediately satisfy such needs, it would be morally and socially unacceptable for us to just help ourselves to other people’s things in order to satisfy our own needs and wants and so later in childhood our Ego comes in to play. The Ego is responsible for dealing with reality and acts to ensure that the impulses of the Id are satisfied in a way that is acceptable to the real world and functions in the conscious, preconscious and unconscious parts of our mind. The reality principle weighs up the pro’s and con’s of an action before deciding whether or not to act upon the impulse. Often the impulses of the Id can be satisfied but through delayed gratification with the Ego allowing the behaviour at an appropriate time and place. The Eg o is a part of the Id that has been somewhat modified and rounded by external factors in the environment in which we live. Freud originally used the word Ego to mean a sense of self but later revised it to represent a set of psychic functions such as judgement, control, intellectual functioning and memory. Finally we develop our Super Ego. The Super Ego is the part of our personality that holds our morals and ideals that we have acquired from our parents and environment and acts as a voice for right and wrong. As with the Ego it is present in the conscious, preconscious and unconscious parts of our mind. The Super Ego consists of two parts, the Ego ideal which sets out the rules and standards for good behaviour. Conformity to behaviours that are approved of by our parents and people in positions of authority give us feelings of pride and accomplishment. The second part of the Ego is the Conscience which holds information on all the things that are viewed as being bad by our parents and the society in which we live. Behaviours that are forbidden or at the very least frowned upon and fill us with feelings of guilt and remorse. The perfection principle of the Super Ego strives to suppress any unacceptable desires of the Id and to make our Ego act upon idealistic rather than realistic standards. One of Freud’s better known theories and also one of the most controversial is that of psycho-sexual development. He proposed that an instinctual libido is present in all of us from birth and develops in five stages. First is the oral stage which occurs from birth up until around the age of eighteen months. The main focus here being the  gratification and pleasures the infant receives through feeding. Children in this stage place objects into their mouths in order to orally explore their environment. At this young age the child is entirely dependent on their carers and thus develops a sense of trust and comfort in relation to those carers. This stage is dominated by the Id as at this point the Ego and Superego have not yet fully developed and all actions are based on the â€Å"Pleasure Principle†. The key experience for a child in the oral stage of development is weaning, allowing the child to become less dependent on their caretakers. Freud said that too much or too little gratification may lead to an oral fixation, which Freud claimed could result in them developing a passive, immature, manipulative personality. This fixation could present in an adult as issues with eating, smoking, nail biting and aggression. The second stage of psycho-sexual development is the anal stage taking place between around 18 months to three years old. Freud believed at this stage that the primary focus of the libido was on controlling bladder and bowel movements. Toilet training is the key experience here which brings into conflict the Id that demands immediate gratification and the Ego that demands delayed gratification. The resulting outcome of this conflict is heavily influenced by the parenting style that a child receives during toilet training. The ideal resolution of this conflict is a gradual adjustment whereby the child adjusts to moderate parental demands, learning the values of physical cleanliness and self control. Freud suggested that if parents over-emphasized toilet training or punished accidents then the child may develop what we term as an anally retentive personality, with the potential to become obsessively concerned with neatness and order. On the other hand if the parents were too lenient then the child may develop an anally expulsive and destructive personality whereby they are self-indulgent, messy and wasteful. Faeces and money are often linked in psychodynamic literature and according to Freudian theory; attitudes to money can reveal what the individual experienced during toilet training. The third stage of psycho-sexual development is the phallic stage taking place between the ages of three and six. During this time a child begins to gain awareness of its body and also the bodies of their parents and other  children, in particular genitalia. They begin to explore their genitals and learn the physical differences between male and female. During this stage boys experience what Freud termed as the Oedipus complex whereby the child wishes to remove his father in order to gain full attention of his mother’s affections. This urge to eliminate the father is controlled by what he termed as castration anxiety and so instead the child learns to imitate the father. Girls experience what Carl Jung in 1913 termed as the Electra complex where instead she wishes to remove the mother in order to gain full possession of her father. Freud however rejected this term as being psycho-analytically inaccurate believing that the reasoning behind the Oedipus complex applied only to male children and that it was wrong to share this analogy between the two sexes. He did however believe that girls experienced what he termed as penis envy and that initially the child experiences a lot of anger towards their mother for not sharing the same appendage as their father but in time they learn to identify with their mother in order to possess their father. Freud was very much influenced by the death of his father in 1896. In the three years following the death of his father, Freud became preoccupied with self-analysis where he realised that he had repressed feelings of anger and resentment against his father. He believed that as a small boy he had been in love with his mother and was jealous of his father. Freud based his theory of early sexual development on personal theory instead of exploring further using empirical methods. The fourth stage of psycho-sexual development is the latency stage taking place from around the age of six up until puberty. During this stage the child enters into a sexually dormant period, consolidating the habits of the previous three stages. The Ego and Superego take precedence over the Id due to the child’s defence mechanisms repressing its instinctual drives during the phallic stage. Now that gratification is delayed, the child is driven to derive pleasure from external activities such as friendships, education and hobbies. Any neuroses established during this fourth stage of psycho-sexual development may be due to the unresolved issues of the Oedipus complex or the Ego’s failure to focus on socially acceptable activities. The fifth and final stage of psycho-sexual development is the genital stage  that spans from puberty throughout the remainder of adult life. As with the phallic stage the genital stage is focussed upon genitalia but in this instance the sexuality is consensual and more often involving another adult in the form of a relationship rather than being solitary and infantile. This is due to the establishment of the Ego which shifts attention away from primary-drive gratification to secondary process thinking and to satisfy desire in a more symbolic and intellectual way through loving relationships, friendships and family. The genital stage is the time when a person is able to resolve any psycho-sexual childhood conflicts that they may have and allows psychological detachment and independence from their parents. In previous stages focus was placed solely on individual needs, now the welfare of others comes strongly into play and if all stages have been completed successfully then Freud believed that the individual should be a well-balanced and fully functioning person. Unfortunately it isn’t all that simple and the Id, the Ego and the Super Ego continuously come into conflict with one another. The Ego has to work to control the demands of the Id whilst at the same time having regard for the restrictions placed upon it by the Super Ego. At times these desires and constraints cause conflict that our Ego is unable to deal with resulting in anxiety and stress. Freud identified three types of anxiety, firstly neurotic anxiety which occurs through fear that we will lose control of the Id’s urges and the resulting punishment for inappropriate behaviour. Secondly reality anxiety which is a fear of external events often culminating in phobias, we are able to reduce such anxiety by avoiding the threatening object or situation. Thirdly is moral anxiety from a fear of violating our own moral principles and values that have been set down by our Super Ego. Neurosis also figured heavily in Freud’s psycho-analytical theory. He proposed that neurosis occurs when the Ego is unable to deal with desires that produce feelings of guilt and a sense of wrong. Through repression these thoughts manifest themselves through symptoms that have no physical dysfunction. The mental illness acts as a replacement for the guilt ridden desires of the Id allowing the Ego to avoid the conflict between itself and the Id. Such symptoms however are worse than the conflict they set out to hide, not only stopping the individual from being accepting of their repressed desires but  also causing them to become socially incapable of enjoying a happy and healthy life. He also believed that neurosis can be triggered by a traumatic childhood event that the individual is unable to handle. Often such experiences give rise to feelings of guilt that we seek to repress through use of various displacement mechanisms. Sometimes these repressed memories make their way back into our conscious minds in a different form producing a great amount of anxiety in turn triggering psychological disorders that seek to block out the real cause. Psychoanalytical therapy has proved productive in being able to help a client uncover unconscious defence mechanisms and help them find better ways of dealing with their anxiety or removing it all together. Psycho-analysis opened up a new view regarding the treatment of mental illness, suggesting that psychological distress could be reduced through talking about their problems with a therapist. The work of Freud was responsible for bringing about a greater understanding of behaviour that was unusual and differences were no longer automatically equated as unacceptable, with understanding comes greater tolerance. He radically changed the view of sexuality making it an acceptable topic of conversation and a natural part of a healthy, happy life. The approach is also credited with highlighting the importance of childhood and our unconscious mind. Despite the influential effect of his theories Freud is open to numerous criticisms. Many psychologists have adopted his ideas but there has been a great deal of modernisation on his original views. Carl Jung who was a pupil of Freud’s even disagreed with certain aspects of his theories; in particular Freud’s reliance on sex as the answer to many problems. Jung went on to develop his own theories known as Analytical Psychology. Erich Fromm rejected Freud’s view that the drives of the human being are solely biological, believing instead that it was down to our freedom of choice and ability to choose our own destiny. He believed any conflict arose as a result of the fear or uncertainty which that freedom entailed. Feminists are particularly critical of the work of Freud due to the sexist nature of many of his ideas. Neo-Freudian Karen Horney proposed that instead of penis envy girls in fact developed power envy and that in their inability to bear  children men develop womb and vagina envy. Scientifically the validity of Freud’s theory of psycho-sexual development is brought in to question due to his perceived personal fixation on human sexuality and the phallic stage of development proved controversial for being based upon clinical observations of the Oedipus complex. Many were critical of the fact that a lot of Freud’s ideas were based on case studies or clinical observations rather than empirical, scientific research. Contemporary criticism questions the universality of Freud’s theory of personality and psycho-sexual development. Anthropologist Bronislaw Malinowski studied the matriarchal society of the Trobriand where young boys are punished by their maternal uncles not their fathers and thus suggested that in this case power is the source of Oedipal conflict not sexual jealousy. Contemporary research has also confirmed that although personality traits corresponding to the oral, anal, phallic, latent and genital stages are observable they are not necessarily fixed stages of childhood or indeed adult personality traits that were derived from childhood. While there is no denying that Freud is of great historical significance and he developed many ground breaking theories and ideas some of which still hold relevance today although many over time have been discredited. It must then be said that Freud’s theory of psychosexual development may in some cases offer us a limited understanding of a client’s issue it would not be ethical practice to rely entirely on this theory when working with a client. Due to its lack of credibility in many areas, Freud’s theory cannot be implemented as a full explanation or means with which to begin the process of understanding and healing. Bibliography Frankland, A. and Sanders, P. 1995. Next steps in counselling. Manchester: PCCS Books. pp.70-76 Hough, M. 2006. Counselling Skills and Theory. 2nd ed. London: Hodder Arnold, pp. 59-86. Malinowski, B. 1927. Sex and repression in savage society. [e-book] London: Routledge and Kegan Paul. http://openlibrary.org/books/OL17967917M/Sex_and_repression_in_savage_society [Accessed: 1st July 2013]. Cherry, K. n.d.. Freud’s Stages of Psychosexual Development. [online] Available at: http://psychology.about.com/od/theoriesofpersonality/ss/psychosexualdev.htm [Accessed: 19 Jun 2013]. Copperwiki.org. 1950. Human Centred Psychotherapy – CopperWiki. [online] Available at: http://www.copperwiki.org/index.php?title=Human_Centred_Psychotherapy [Accessed: 01 Jul 2013] En.wikipedia.org. 2013. Psychosexual development – Wikipedia, the free encyclopedia. [online] Available at: http://en.wikipedia.org/wiki/Psychosexual_development [Accessed: 01 Jul 2013]. http://highered.mcgraw-hill.com/sites/dl/free/0072969806/286620/fei69806_ch02

Martin Luther King V. Malcolm X

Alan mason Period 8 Martin Luther major power v. Malcolm X Martin Luther nance has been known for utilize heartsease to help him suffer compare among alone mountain but especially Afri feces-Americans. He was non the type of leader that resorted to delirium in order to get the reachity that he fought for. fag has been known for sorrowful stack by the use of his speeches standardised Kings I Have a day-dream and Vie Been to the Mountaintop speeches. King shows through with(predicate) these speeches that plain though he was monishmined to get equal fulls for every black, he as only handout to stick to subtle, peaceful ship canal homogeneous court cases, hold out, and boycotts.This ism is the exact oppo flummoxe to Malcolm X, an new(prenominal) civic Rights Leader, who thought that African American should non waste their time on protest and boycotts (Document 7). Malcolm, like King, was not in upgrade of fury but believe that African Americans should by a ll meaner use violence to protect themselves when they attacked by racists (Document 9). But, Martin Luther Kings doctrine was much unspoiled for the African-American familiarity consequently Malcolm Axs because the try that he put Into his nonviolent philosophy made gaining rights to a greater extent lucky and more meaningful.Martin Luther King showed that he had the check philosophy compared to Malcolm X because of his stance on violence. One way King upheld his beliefs was through protest. King verbalise In his Our beau ideal Is Marching speech, We are on the shine now. The burning of our churches will not deter us. We are on the move now. The bomb of our homes will not dissuade us (Document 4). So Protesters, Including King himself were having their homes burned subject for retesting for what they believe Is right. In March of 1965 King and other protesters walked In order to overdress awareness of the need for a right to vote Right Act. This Act was passed later that year.But dismantle though they achieved their goal for voting right does not mean that the protesters did not induce opposition for their actions. Mass arrest, police attacks, and body politic troopers blockading all occurred throughout the walk. If King chose to fight back then that could gain either delayed getting the voting Right Act approved, or It whitethorn take a shit been felt that African-Americans did not deserve to get the Act approved. But King Just showed by obtaining from violence you can still get what you loss. Martin Luther King showed that thither were other ways to fight for what you want aside form fighting Itself.King did this by using boycotts and strikes. He said, There Is zero quite so effective as refusal to cooperate economically with the forces and Institutions. King recognise that one of the ways he was deprivation to get American to give them the rights that African-Americans treasured was by disrupting the flow of money that was going Into the Caucasian shops. This meaner by not purchase from places that o not offer Jobs for African-Americans then those businesses would see how much they needed the African-American community, even If It meant having to hire African- American workers.Malcolm X did not agree with boycotts and strikes he said, We have to learn how to own and operate the businesses of our community and develop to be involved in picketing and boycotting other people in other communities in order to get a Job (Document 7). But this idea completely went against the philosophy of being together not separate. If this would have happened then the country would eave ever been separate and things would have never been equal.Martin Luther top executive has been known for using his words instead of his fist when it comes to defending what he legion(predicate) thousands of others believed to be correct. King once said in his I Have a Dream speech that he had a dream that former grave workers and the sons of former slave owners will be able to sit down together at the shelve of brotherhood They will not be Judged by the color of their skin but by their character We will be able to work together, pray together, to effort together, to go o Jail together, to climb up for freedom together (Document 2).King fought for African Americans rights by using his words. He told the people of his desires for and equal and fair life where people could live in harmony with each(prenominal) other and not be judged by their skin tone. He thought of a time where there would be no more separate but equal as were stated prior, people would be able to work together And go to Jail together. (Document 2) So by using his speeches where thousands of people came to listen to him talk, he locomote a nation to be in favor of his reasonings.In conclusion, Martin Luther Kings philosophy was more beneficial for the African- American community then Malcolm Axs because the effort that he put into his nonviolen t philosophy made gaining rights more successful and more meaningful. King kept up with his no violence idea by dynamic in other things that got Americas attention like protest, boycotts and speeches. King strives to achieve equality and because of his hard efforts he has succeeded in more ways than one to help African-Americans get the same civil rights as Caucasian Americans.